Trap-TRAP, a Versatile Tool for Tissue-Specific Translatomics in Zebrafish

Trap-TRAP, a Versatile Tool for Tissue-Specific Translatomics in Zebrafish

Blog Article

Developmental and physiological processes depend on the transcriptional and translational activity of heterogeneous cell populations.A main challenge in gene expression studies is dealing with this intrinsic complexity while keeping sequencing efficiency.Translating ribosome affinity purification (TRAP) methods have allowed cell-specific recovery of polyribosome-associated RNAs by genetic tagging of ribosomes in selected cell populations.Here we combined the TRAP approach with adapted enhancer trap methods (trap-TRAP) to systematically generate Battery zebrafish transgenic lines suitable for tissue-specific translatome interrogation.

Through the random integration of a GFP-tagged version of the large subunit ribosomal protein L10a (EGFP-Rpl10a), we have generated stable lines driving expression in a variety of tissues, including the retina, 3 Piece Reclining Sectional with Chaise skeletal muscle, lateral line primordia, rhombomeres, or jaws.To increase the range of applications, a UAS:TRAP transgenic line compatible with available Gal4 lines was also generated and tested.The resulting collection of lines and applications constitutes a resource for the zebrafish community in developmental genetics, organ physiology and disease modelling.